Journal: World Journal of Gastroenterology : WJG

Article Title: Proteome-based biomarkers in pancreatic cancer

doi: 10.3748/wjg.v17.i44.4845

Figure Lengend Snippet: A selection of potential biomarkers for pancreatic cancer

Article Snippet: In this review, we focus on potential proteome-based biomarkers to be used in pancreatic cancer (Table ), hopefully indicative for further research within the field. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Potential biomarkers for pancreatic cancer Expression Proteomic tools Ref. Tissue 14-3-3 sigma + 2-D SDS PAGE, MS [13] Annexin A4 + 2-D SDS PAGE, MS [13] Anterior gradient 2 + 2-D SDS PAGE, MS [13] AOX1 - PowerBlot [14] ATP7A + PowerBlot [14] Biglycan + SDS-PAGE, LC-MS/MS [22] Galectin-1 + 2-D SDS PAGE, MS [9] Galectin-3 + MALDI-TOF-MS [7] Gelsolin - Proteomic chip [23] Major vault protein + 2-D SDS PAGE, MS [13] Pigment epithelium-derived factor + SDS-PAGE, LC-MS/MS [22] Plectin-1 + Western blotting [16] S100A6 + 2-D SDS PAGE, MS [11] S100A8 (stroma) + 2DE, LC-MS/MS [12] S100A9 (stroma) + 2DE, MALDI-TOF-MS [12] S100A10 + 2-D SDS PAGE, MS [13] S100A11 + MALDI-TOF-MS [7] Thrombospondin-2 + SDS-PAGE, LC-MS/MS [22] βIGH3 + SDS-PAGE, LC-MS/MS [22] UHRF1 + PowerBlot [14] Serum/plasma α-1B-glycoprotein precursor + DIGE, MS/MS [38] Anterior gradient 2 + iTRAQ, MS/MS [18] Apolipoprotein A-II - SELDI-TOF, MS [24] Apolipoprotein C-I + SELDI-TOF, MS [24] Caldecrin - ICAT, MS [43] CXCL 7 - LC-MS [29] DJ-1 + DIGE, MS/MS [38] Fibrinogen β chain ICAT, MS [43] HSP27 + Protein-chip technology [33] Pancreatic juice Lithostathine I α - 2DE, MALDI-TOF-MS [40] Matrix metalloproteinase-9 + DIGE, MS/MS [38] Neural cell adhesion molecule L1 + ICAT, MS [43] p-Akt + Bio-Plex suspension array [32] p-ERK1/2 + Bio-Plex suspension array [32] Phosphor-cAMP response element binding protein + Bio-Plex suspension array [32] Phosphor-p90 ribosomal S6 kinase + Bio-Plex suspension array [32] p-IκB-α + Bio-Plex suspension array [32] Plasminogen + ICAT, MS [43] Platelet factor 4 - MALDI-TOF [31] p-MEK1 + Bio-Plex suspension array [32] Transthyretin + 2DE, MALDI-TOF-MS [45] Urine Annexin A2 - 2-D SDS PAGE [46] CD59 - 2-D SDS PAGE [46] Gelsolin - 2-D SDS PAGE [46] Cell lines Apoprotein E + SILAC [48] Cadherin Not in metastatic tumor cell LC-MS/MS [52] Catenin Not in metastatic tumor cell LC-MS/MS [52] CD9 + SILAC [48] Fibronectin receptor + SILAC [48] Galectin Not in primary tumor cell LC-MS/MS [52] Glucagon + Protein array [53] Integrin Not in metastatic tumor cell LC-MS/MS [52] Perlecan + LC-MS/MS [48] Prolactin - Protein array [53] Open in a separate window SILAC: Stable isotope labelling with amino acids in cell culture; DIGE: Difference gel electrophoresis; MS: Mass spectrometry; iTRAQ: Isobaric tags for relative and absolute quantification; ICAT: Isotope-coded affinity tag; βIGH3: Ig-h3 precursor; A1BG: α-1B-glycoprotein precursor; +: Up-regulated; –: Down-regulated expression in pancreatic cancer as compared with controls.

Techniques: Selection, SDS Page, Western Blot, Multiplex sample analysis, Suspension, Binding Assay, Protein Array